Adaptation of the State Trait Anxiety Inventory in Arabic: A Comparison with the American STAI

The main goal of the present study was to develop an Arabic adaptation of the State Trait Anxiety Inventory (STAI, Form Y, Spielberger, 1983). In addition, cultural and linguistic influences on the experience and expression of anxiety were assessed. The American STAI and fifty initial Arabic items were administered to 286 university students at the American University of Beirut, Lebanon. The American STAI was also administered to 336 university students at the University of South Florida. Item and factor analyses were conducted on responses of the calibration sample to obtain the final set of Arabic items, which was validated using the responses of the validation sample. In conducting item selection and validation of the Arabic STAI, internal consistency coefficients for subscales, corrected item-total correlations, alpha coefficients if-item-deleted, item-factor loadings, and theoretical meaningfulness were all used as criteria for selection of the best 10 Arabic items to be included in each subscale of the STAI: S-Anxiety Absent, S-Anxiety Present, T-Anxiety Absent, T-Anxiety present. The two-factor solution for the Arabic STAI yielded a simple solution with two distinct factors: Anxiety Present and Anxiety Absent for each of S-Anxiety and T-Anxiety, lending more support to the theoretical distinction of state and trait anxiety. Lebanese students reported significantly higher anxiety levels than their American peers on S-Anxiety Present, T-Anxiety Absent, and T-Anxiety Present, S-Anxiety and T-Anxiety of the American STAI. For S-Anxiety Absent, scores for the Lebanese sample were lower than American students but did not reach significance levels. S-Anxiety Absent and T-Anxiety Absent subscales assessed lower levels of anxiety rather than the higher levels of anxiety assessed by S-Anxiety Present and T-Anxiety Present. Females tend to experience and express higher levels of mild and severe anxiety symptoms as compared to males in both samples. Factor analyses of the American STAI for the American and Lebanese samples revealed similar two and three- factor solutions. For each of the State and trait subscales, three factors emerged: Anxiety Absent, Worry, and Emotionality factors, denoting the importance of cognitions and feelings in the experience and expression of anxiety

Key Education Issues in Review: Nevada Academic Content Standards

final_soap_GNV120025.fa: Assembly of Hemiargus ceraunus from Genbank SRA accession #SRR1299274, using multiple kmers (13,23,33,43,63) with SOAPdenovo-Trans v1.01. Different Kmer assemblies were combined with cd-hit-est and processed with the fastx toolkit. See Kawahara and Breinholt (2014) for more details

Phylogenetic evidence from freshwater crayfishes that cave adaptation is not an evolutionary dead-end.

Caves are perceived as isolated, extreme habitats with a uniquely specialized biota, which long ago led to the idea that caves are evolutionary dead-ends. This implies that cave-adapted taxa may be doomed for extinction before they can diversify or transition to a more stable state. However, this hypothesis has not been explicitly tested in a phylogenetic framework with multiple independently evolved cave-dwelling groups. Here, we use the freshwater crayfish, a group with dozens of cave-dwelling species in multiple lineages, as a system to test this hypothesis. We consider historical patterns of lineage diversification and habitat transition as well as current patterns of geographic range size. We find that while cave-dwelling lineages have small relative range sizes and rarely transition back to the surface, they exhibit remarkably similar diversification patterns to those of other habitat types and appear to be able to maintain a diversity of lineages through time. This suggests that cave adaptation is not a dead-end for freshwater crayfish, which has positive implications for our understanding of biodiversity and conservation in cave habitats

An Earthworm Riddle: Systematics and Phylogeography of the Spanish Lumbricid Postandrilus

As currently defined, the genus Postandrilus Qui and Bouché, 1998, (Lumbricidae) includes six earthworm species, five occurring in Majorca (Baleares Islands, western Mediterranean) and another in Galicia (NW Spain). This disjunct and restricted distribution raises some interesting phylogeographic questions: (1) Is Postandrilus distribution the result of the separation of the Baleares-Kabylies (BK) microplate from the proto-Iberian Peninsula in the Late Oligocene (30-28 Mya)--vicariant hypothesis? (2) Did Postandrilus diversify in Spain and then colonize the Baleares during the Messinian salinity crisis (MSC) 5.96-5.33 Mya--dispersal hypothesis? (3) Is the distribution the result of a two-step process--vicariance with subsequent dispersal?To answer these questions and assess Postandrilus evolutionary relationships and systematics, we collected all of the six Postandrilus species (46 specimens - 16 locations) and used Aporrectodea morenoe and three Prosellodrilus and two Cataladrilus species as the outgroup. Regions of the nuclear 28S rDNA and mitochondrial 16S rDNA, 12S rDNA, ND1, COII and tRNA genes (4,666 bp) were sequenced and analyzed using maximum likelihood and Bayesian methods of phylogenetic and divergence time estimation. The resulting trees revealed six new Postandrilus species in Majorca that clustered with the other five species already described. This Majorcan clade was sister to an Iberian clade including A. morenoe (outgroup) and Postandrilus bertae. Our phylogeny and divergence time estimates indicated that the split between the Iberian and Majorcan Postandrilus clades took place 30.1 Mya, in concordance with the break of the BK microplate from the proto-Iberian Peninsula, and that the present Majorcan clade diversified 5.7 Mya, during the MSC.Postandrilus is highly diverse including multiple cryptic species in Majorca. The genus is not monophyletic and invalid as currently defined. Postandrilus is of vicariant origin and its radiation began in the Late Oligocene

A global phylogeny of butterflies reveals their evolutionary history, ancestral hosts and biogeographic origins

Butterflies are a diverse and charismatic insect group that are thought to have evolved with plants and dispersed throughout the world in response to key geological events. However, these hypotheses have not been extensively tested because a comprehensive phylogenetic framework and datasets for butterfly larval hosts and global distributions are lacking. We sequenced 391 genes from nearly 2,300 butterfly species, sampled from 90 countries and 28 specimen collections, to reconstruct a new phylogenomic tree of butterflies representing 92% of all genera. Our phylogeny has strong support for nearly all nodes and demonstrates that at least 36 butterfly tribes require reclassification. Divergence time analyses imply an origin similar to 100 million years ago for butterflies and indicate that all but one family were present before the K/Pg extinction event. We aggregated larval host datasets and global distribution records and found that butterflies are likely to have first fed on Fabaceae and originated in what is now the Americas. Soon after the Cretaceous Thermal Maximum, butterflies crossed Beringia and diversified in the Palaeotropics. Our results also reveal that most butterfly species are specialists that feed on only one larval host plant family. However, generalist butterflies that consume two or more plant families usually feed on closely related plants

getlist

python script to get list of sequences from a fasta fil

ortholog_filter

python script to process the output of BLAST to find if the location of the best hit on the genome is the same location as the probe target from that genome

contamination_filter

python script to process blast results of blasting sequences from each loci against themselves using usearch to identify contaminatio

IBA

python script to assemble AHE data loci by loc

counting_monster

python script to count the loci per taxa and put into a tab separated matri